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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">radioelectronics</journal-id><journal-title-group><journal-title xml:lang="ru">Известия высших учебных заведений России. Радиоэлектроника</journal-title><trans-title-group xml:lang="en"><trans-title>Journal of the Russian Universities. Radioelectronics</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1993-8985</issn><issn pub-type="epub">2658-4794</issn><publisher><publisher-name>Saint Petersburg Electrotechnical University</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.32603/1993-8985-2022-25-6-40-49</article-id><article-id custom-type="elpub" pub-id-type="custom">radioelectronics-693</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ТЕЛЕВИДЕНИЕ И ОБРАБОТКА ИЗОБРАЖЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>TELEVISION AND IMAGE PROCESSING</subject></subj-group></article-categories><title-group><article-title>Автоматический метод сегментации флуоресцентных изображений, полученных в ближнем инфракрасном диапазоне</article-title><trans-title-group xml:lang="en"><trans-title>Automatic Method for Segmentation of Fluorescent Images Obtained in the Near-Infrared Region</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Обухова</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Obukhova</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Обухова Наталия Александровна – доктор технических наук (2009), зав. кафедрой телевидения и видеотехники</p><p>ул. Профессора Попова, д. 5 Ф, Санкт-Петербург, 197022</p></bio><bio xml:lang="en"><p>Nataliia A. Obukhova, Dr Sci. (Eng.) (2009), Head of Television and Video Equipment Department</p><p>5 F, Professor Popov St., St Petersburg 197022</p></bio><email xlink:type="simple">naobukhova@etu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ян</surname><given-names>Синь</given-names></name><name name-style="western" xml:lang="en"><surname>Yang</surname><given-names>Xin</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ян Синь – магистр по направлению "Радиотехника" (2020), аспирант кафедры телевидения и видеотехники</p><p>ул. Профессора Попова, д. 5 Ф, Санкт-Петербург, 197022</p></bio><bio xml:lang="en"><p>Xin Yang, Master on Radio Engineering (2020), Postgraduate Student at Television and Video Equipment Department</p><p>5 F, Professor Popov St., St Petersburg 197022</p></bio><email xlink:type="simple">877355442@qq.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Санкт-Петербургский государственный электротехнический университет "ЛЭТИ" им. В. И. Ульянова (Ленина)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Saint Petersburg Electrotechnical University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>28</day><month>12</month><year>2022</year></pub-date><volume>25</volume><issue>6</issue><fpage>40</fpage><lpage>49</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Обухова Н.А., Ян С., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Обухова Н.А., Ян С.</copyright-holder><copyright-holder xml:lang="en">Obukhova N.A., Yang X.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://re.eltech.ru/jour/article/view/693">https://re.eltech.ru/jour/article/view/693</self-uri><abstract><sec><title>Введение</title><p>Введение. В настоящее время технология флуоресцентной визуализации в ближнем инфракрасном диапазоне широко применяется при проведении лапароскопических операций. Основой технологии является сегментация области флуоресценции на изображениях, полученных в ближнем инфракрасном диапазоне (БИК-изображениях). Для повышения качества и эффективности навигации необходимо разработать автоматический метод, позволяющий сегментировать флуоресцентные области на БИК-изображениях с максимальной точностью.</p></sec><sec><title>Цель работы</title><p>Цель работы. Повышение точности автоматической сегментации флуоресцентных изображений, полученных в ближнем инфракрасном диапазоне.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Предложенный метод состоит из двух этапов. На первом этапе выполняется предварительная сегментация изображения на основе адаптивного порога, найденного по методу Оцу. На втором этапе сегментированная область уточняется с помощью взвешенного метода Оцу. Главной особенностью метода является автоматическое определение параметра α, являющегося ключевым для эффективной работы взвешенного метода Оцу. Экспериментальное исследование метода было выполнено на реальных лапароскопических изображениях, общее число изображений в исследовании – 276. Значение ошибки сегментации (метрика ME – misclassification error) было использовано для оценки качества работы предложенного метода.</p></sec><sec><title>Результаты</title><p>Результаты. Среднее значение ошибки сегментации (метрика ME) предложенного метода составляет 10.4 %, а традиционного метода Оцу – 27.1%.</p></sec><sec><title>Заключение</title><p>Заключение. По сравнению с традиционным методом Оцу использование разработанного метода позволяет повысить точность сегментации флуоресцентных изображений. Это обеспечивает высокую чувствительность и специфичность при проведении диагностики и позволяет реализовать более эффективную навигацию в процессе лапароскопической операции.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. Near-infrared fluorescence imaging technology is widely used in laparoscopic surgery. Intraoperative fluorescence navigation is based on accurate segmentation of fluorescent regions in near-infrared images (NIR images), thus increasing the accuracy and safety of surgical intervention. Moreover, it is an important auxiliary technology for laparoscopic surgery. Therefore, the search for an automatic method that allows for accurate segmentation of fluorescent regions in NIR images can contribute to an improved efficiency of intraoperative navigation.</p></sec><sec><title>Aim</title><p>Aim. Development of a method for automatic segmentation of fluorescent images obtained in the near infrared range.</p></sec><sec><title> Materials and methods</title><p> Materials and methods. The proposed method consists of two stages. At the first stage, a preliminary segmentation of the image is performed based on the adaptive threshold found by Otsu’s method. At the second stage, the segmented area is refined using Otsu’s weighted method. The main advantage of the proposed method consists in the automatic determination of parameter α, which determines the performance of Otsu’s weighted method. Experiments were carried out using 276 actual laparoscopic images. The metric misclassification error (ME) was used to assess the quality of segmentation.</p></sec><sec><title>Results</title><p>Results. The average ME of the proposed method was found to be 10.4 %, compared to that obtained by the conventional Otsu’s method of 27.1 %.</p></sec><sec><title>Conclusion</title><p>Conclusion. In comparison with Otsu’s method, the developed method shows an increased efficiency and accuracy of fluorescent image segmentation. This allows for a higher diagnostic accuracy and a more efficient navigation during laparoscopic surgery.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>автоматическая сегментация</kwd><kwd>пороговая сегментация</kwd><kwd>метод Оцу</kwd><kwd>флуоресцентные  лапароскопические изображения</kwd><kwd>цифровая обработка изображений</kwd></kwd-group><kwd-group xml:lang="en"><kwd>automatic segmentation</kwd><kwd>threshold segmentation</kwd><kwd>Otsu’s method</kwd><kwd>fluorescent laparoscopic images</kwd><kwd>digital image processing</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование проводится при поддержке Государственного комитета по стипендиям КНР (грант № 202009010036).</funding-statement><funding-statement xml:lang="en">The study was realized with the support of the China Scholarship Council (grant no. 202009010036).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Zhu B., Sevick-Muraca E. M. A review of performance of near-infrared fluorescence imaging devices used in clinical studies // The British J. of Radiology. 2015. Vol. 88, № 1045. P. 20140547. doi: 10.1259/bjr.20140547</mixed-citation><mixed-citation xml:lang="en">Zhu B., Sevick-Muraca E. M. A Review of Performance of Near-Infrared Fluorescence Imaging Devices Used in Clinical Studies. The British J. of Radiology. 2015, vol. 88, no. 1045, p. 20140547. doi: 10.1259/bjr.20140547</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Clinical applications of indocyanine green (ICG) enhanced fluorescence in laparoscopic surgery / L. Boni, G. David, A. Mangano, G. Dionigi, S. Rausei, S. Spampatti, E. Cassionotti, A. Fingerhut // Surgical Endoscopy. 2015. Vol. 29, № 7. P. 2046–2055. doi: 10.1007/s00464-014-3895-x</mixed-citation><mixed-citation xml:lang="en">Boni L., David G., Mangano A., Dionigi G., Rausei S., Spampatti S., Cassionotti E., Fingerhut A. Clinical Applications of Indocyanine Green (ICG) Enhanced Fluorescence in Laparoscopic Surgery. Surgical Endoscopy. 2015, vol. 29, no. 7, pp. 2046–2055. doi: 10.1007/s00464-014-3895-x</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">The clinical use of indocyanine green as a near‐infrared fluorescent contrast agent for image‐guided oncologic surgery / B. E. Schaafsma, J. S. D. Mieog, M. Hutteman, J. R. Vorst, P. J. K. Kuppen, C. W. G. M. Löwik, J. V. Frangioni, C. J. H. Velde, A. L. Vahrmeijer // J. of Surgical Oncology. 2011. Vol. 104, № 3. P. 323–332. doi: 10.1002/jso.21943</mixed-citation><mixed-citation xml:lang="en">Schaafsma B. E., Mieog J. S. D., Hutteman M., Vorst J. R., Kuppen P. J. K., Löwik C. W. G. M., Frangioni J. V., Velde C. J. H., Vahrmeijer A. L. The Clinical Use of Indocyanine Green as a Near – Infrared Fluorescent Contrast Agent for Image – Guided Oncologic Surgery. J. of Surgical Oncology. 2011, vol. 104, no. 3, pp. 323–332. doi: 10.1002/jso.21943</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Real-time navigation for liver surgery using projection mapping with indocyanine green fluorescence: development of the novel medical imaging projection system / H. Nishino, E. Hatano, S. Seo, T. Nitta, T. Saito, M. Nakamura, K. Hattori, M. Takatani, H. Fuji, K. Taura, Sh. Uemoto // Annals of surgery. 2018. Vol. 267, № 6. P. 1134–1140. doi: 10.1097/SLA.0000000000002172</mixed-citation><mixed-citation xml:lang="en">Nishino H., Hatano E., Seo S., Nitta T., Saito T., Nakamura M., Hattori K., Takatani M., Fuji H., Taura K., Uemoto Sh. Real-Time Navigation for Liver Surgery Using Projection Mapping with Indocyanine Green Fluorescence: Development of the Novel Medical Imaging Projection System. Annals of Surgery. 2018, vol. 267, no. 6, pp. 1134–1140. doi: 10.1097/SLA.0000000000002172</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Near-infrared fluorescence imaging in humans with indocyanine green: a review and update / M. V. Marshall, J. C. Rasmussen, I.-Ch. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, E. M. Sevick-Muraca // The Open Surgical Oncology J. 2010. Vol. 2, № 2. P. 12–25. doi: 10.2174/1876504101002010012</mixed-citation><mixed-citation xml:lang="en">Marshall M. V., Rasmussen J. C., Tan I.-Ch., Aldrich M. B., Adams K. E., Wang X., Fife C. E., Maus E. A., Smith L. A., Sevick-Muraca E. M. Near-Infrared Fluorescence Imaging in Humans with Indocyanine Green: a Review and Update. Open Surgical Oncology J. 2010, vol. 2, no. 2, pp. 12–25. doi: 10.2174/1876504101002010012</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Bali A., Singh S. N. A review on the strategies and techniques of image segmentation // 5th Intern. Conf. on Advanced Computing &amp; Communication Technologies, Haryana, India, 21–22 Feb. 2015. Piscataway: IEEE, 2015. P. 113–120. doi: 10.1109/ACCT.2015.63</mixed-citation><mixed-citation xml:lang="en">Bali A., Singh S. N. A Review on the Strategies and Techniques of Image Segmentation. 5th Intern. Conf. on Advanced Computing &amp; Communication Technologies, Haryana, India, 21–22 Feb. 2015. Piscataway, IEEE, 2015, pp. 113–120. doi: 10.1109/ACCT.2015.63</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Qiao W., Wu C. Weighting Otsu's Segmentation Method and Its Fuzzy Theory Explanation. Computer Engineering. 2009. Vol. 10. P. 211–213. doi: 10.3969/j.issn.1000-3428.2009.10.070 (In Chinese)</mixed-citation><mixed-citation xml:lang="en">Qiao W, Wu C. Weighting Otsu's Segmentation Method and Its Fuzzy Theory Explanation. Computer Engineering. 2009, vol. 10, pp. 211–213. doi: 10.3969/j.issn.1000-3428.2009.10.070 (in Chinese)</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Yuan X., Wu L., Peng Q. An improved Otsu method using the weighted object variance for defect detection // Applied surface science. 2015. Vol. 349. P. 472–484. doi: 10.1016/j.apsusc.2015.05.033</mixed-citation><mixed-citation xml:lang="en">Yuan X., Wu L., Peng Q. An Improved Otsu Method Using the Weighted Object Variance for Defect Detection. Applied Surface Science. 2015, vol. 349, pp. 472–484. doi: 10.1016/j.apsusc.2015.05.033</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang J., Hu J. Image segmentation based on 2D Otsu method with histogram analysis // Intern. Conf. on computer science and software engineering, Wuhan, China, 12–14 Sept. 2008. Piscataway: IEEE, 2008. Vol. 6. P. 105–108. doi: 10.1109/CSSE.2008.206</mixed-citation><mixed-citation xml:lang="en">Zhang J., Hu J. Image Segmentation Based on 2D Otsu Method with Histogram Analysis. Intern. Conf. on Computer Science and Software Engineering. Wuhan, China, 12–14 Sept. 2008. Piscataway, IEEE, 2008, vol. 6, pp. 105–108. doi: 10.1109/CSSE.2008.206</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">A multi-scale 3D Otsu thresholding algorithm for medical image segmentation / Y. Feng, H. Zhao, X. Li, X. Zhang, H. Li // Digital Signal Processing. 2017. Vol. 60. P. 186–199. doi: 10.1016/j.dsp.2016.08.003</mixed-citation><mixed-citation xml:lang="en">Feng Y., Zhao H., Li X., Zhang X., Li H. A Multi-Scale 3D Otsu Thresholding Algorithm for Medical Image Segmentation. Digital Signal Processing. 2017, vol. 60, pp. 186–199. doi: 10.1016/j.dsp.2016.08.003</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Salem N., Malik H., Shams A. Medical image enhancement based on histogram algorithms // Procedia Computer Science. 2019. Vol. 163. P. 300–311. doi: 10.1016/j.procs.2019.12.112</mixed-citation><mixed-citation xml:lang="en">Salem N., Malik H., Shams A. Medical Image Enhancement Based on Histogram Algorithms. Procedia Computer Science. 2019, vol. 163, pp. 300–311. doi: 10.1016/j.procs.2019.12.112</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Otsu N. A threshold selection method from gray-level histograms // IEEE transactions on systems, man, and cybernetics. 1979. Vol. 9, № 1. P. 62–66. doi: 10.1109/TSMC.1979.4310076</mixed-citation><mixed-citation xml:lang="en">Otsu N. A Threshold Selection Method from Gray-Level Histograms. IEEE Transactions on Systems, Man, and Cybernetics. 1979, vol. 9, no. 1, pp. 62–66. doi: 10.1109/TSMC.1979.4310076</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Sezgin M., Sankur B. Survey over image thresholding techniques and quantitative performance evaluation // J. of Electronic imaging. 2004. Vol. 13, № 1. P. 146–165. doi: 10.1117/1.1631315</mixed-citation><mixed-citation xml:lang="en">Sezgin M., Sankur B. Survey over Image Thresholding Techniques and Quantitative Performance Evaluation. J. of Electronic Imaging. 2004, vol. 13, no. 1, pp. 146–165. doi: 10.1117/1.1631315</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Clinical development and potential of photo-thermal and photodynamic therapies for cancer / X. Li, J. F. Lovell, J. Yoon, X. Chen // Nature Reviews Clinical Oncology. 2020. Vol. 17, № 11. P. 657–674. doi: 10.1038/s41571-020-0410-2</mixed-citation><mixed-citation xml:lang="en">Li X., Lovell J. F., Yoon J., Chen X. Clinical Development and Potential of Photothermal and Photo-dynamic Therapies for Cancer. Nature Reviews Clinical Oncology. 2020, vol. 17, no. 11, pp. 657–674. doi: 10.1038/s41571-020-0410-2</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Photodynamic therapy for management of cervical intraepithelial neoplasia II and III in young patients and obstetric outcomes / M. C. Choi, S. G. Jung, H. Park, S. Y. Lee, C. Lee, Y. Y. Hwang, S. J. Kim // Lasers in Surgery and Medicine. 2013. Vol. 45, № 9. P. 564–572. doi: 10.1002/lsm.22187</mixed-citation><mixed-citation xml:lang="en">Choi M. C., Jung S. G., Park H., Lee S. Y., Lee C., Hwang Y. Y., Kim S. J. Photodynamic Therapy for Management Of Cervical Intraepithelial Neoplasia II and III in Young Patients and Obstetric Outcomes. Lasers in Surgery and Medicine. 2013, vol. 45, no. 9, pp. 564–572. doi: 10.1002/lsm.22187</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Отдельнова О. Б., Хашукоева А. З., Ибрагимова М. И. Возможности фотодинамической терапии с использованием фотосенсибилизатора фотодитазин в лечении гинекологических заболеваний // Российский биотерапевтический журн. 2008. Т. 7, № 4. С. 47–52.</mixed-citation><mixed-citation xml:lang="en">Otdelnova O. B., Khashukoeva A. Z., Ibragimova M. I. Photodynamic therapy with photodytazin in treatment of gynecologic diseases. Russian J. of Bio-therapy. 2008, vol. 7, no. 4, pp. 47–52. (In Russ.)</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
